Figure 1

Illustrates CYP21A2 gene mutations detected by allele-specific PCR (Ia) fragment A (Ib) Pro31Leu (Ic) I2splice (IIa) fragment B (IIb) Ileu173Asn (IIc) Gln319Stop, (IId) Arg357Trp (IIf) Val238Glu, (IIe) Leu308insT. ϕX174 and 50 bp DNA markers were used for fragment size estimation.