Figure 1

RT-PCR and dot-blot hybridization for GATA-6, GATA-4, and LHR expression in normal human adrenal and in NCI-H295A cells. Correct sizes for GATA-6 and GATA-4 transcripts are, respectively, 285 and 194 bp. M: 1 kb molecular size ladder Ad: normal adrenal. NCI: NCI-H295A cells. +: genomic DNA. -: negative control for RT-PCR.