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Figure 3 | BMC Endocrine Disorders

Figure 3

From: The uncoupling protein 1 gene, UCP1, is expressed in mammalian islet cells and associated with acute insulin response to glucose in African American families from the IRAS Family Study

Figure 3

UCP1 and insulin co-localize in mammalian islet cells. Pancreas sections from monkey (Macaca fascicularis), human, and mouse (indicated at left, together with magnification) were stained using immunohistochemistry with anti-UCP1 (left); anti-Insulin (middle); or double-stained with anti-UCP1 and anti-Insulin (right). Sections stained with anti-UCP1 were visualized with Vector Red, resulting in pink staining of the islet cells, seen in the left panel for each species. Gal-X visualization of anti-Insulin produced a light blue stain of islet cell cytoplasm, seen in the middle panel for each species. Co-localization (overlay) of both anti-UCP1 (pink) and anti-Insulin (light blue) stains produced the purple staining of the islets seen in the third panel for each species. Examples of islet cells with cytoplasmic staining for the proteins of interest are indicated by the arrows.

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BMC Endocrine Disorders

ISSN: 1472-6823

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