Fig. 2

Over-expression of miR-146a-3p down-regulated the expression of PIWIL2 in TC cell lines

(A) The mRNA level of miR-146a-3p in normal thyroid follicular epithelial cells and various TC cell lines. RNU6B was used for normalization. (B) The mRNA level of PIWIL2 in normal thyroid follicular epithelial cells and various TC cell lines. GAPDH was used for normalization. (C) The mRNA level of PIWIL2 in TPC-1 cell lines after miR-146a-3p was over-expressed or knocked down. (D) The mRNA level of miR-146a-3p in TPC-1 cell lines after miR-146a-3p was over-expressed or knocked down. E, F. The protein expression of PIWIL2 in TPC-1 cell lines after miR-146a-3p was over-expressed or knocked down. GAPDH was used for normalization. *: P < 0.05 compared to NTHY-ORI 3 − 1; **: P < 0.01 compared to NTHY-ORI 3 − 1; ^##: P < 0.01 between groups. N = 6. TC: thyroid cancer