Sex hormones differently regulate lipid metabolism genes in primary human hepatocytes

BMC Endocrine Disorders

Table 2 LC-MS analytics of sex hormones in adherent PHH cultures

Sample	AUC of E2	Metabolized E2 after 24 h [%]	AUC of T	Metabolized T after 24 h [%]	AUC of P	Metabolized P after 24 h [%]
NC	419	-	29,667	-	46,289	-
FD1	ND	100	6441	96.9	3071	99.6
FD2	ND	100	3994	98.3	1782	99.8
FD3	ND	100	2349	98.8	1223	99.8
FD4	ND	100	3327	98.5	895	99.9
FD5	ND	100	2762	98.4	1004	99.8
MD1	ND	100	5983	98.0	562	99.9
MD2	ND	100	2870	98.8	2101	99.7
MD3	ND	100	5216	98.0	787	99.9
MD4	ND	100	2272	98.8	730	99.9
MD5	ND	100	3413	98.3	868	99.9

Primary human hepatocytes (PHHs) were cultured with one of the respective sex hormones for 24 h. Cell culture media prior to cell culture were used as negative controls. Values represent integrated peak areas (area under the curve; AUC) for the parent of the respective hormone normalized to the AUC of its respective isotopic standard. The amount of hormone metabolized after 24 h is given as percentage of the respective hormone value in relation to its negative control value
Abbreviations E2, 17β-estradiol; T, testosterone; P, progesterone

ISSN: 1472-6823